Cross-Linked Enzyme Aggregate (CLEA) Preparation from Waste Activated Sludge

Enzymes are used extensively as industrial bio-catalysts in various manufacturing and processing sectors. However, commercial enzymes expensive part due to the high cost of nutrient medium for biomass culture. Activated sludge (AS) is a waste product biological wastewater treatment consists microbial that degrades organic matter by producing substantial quantities hydrolytic enzymes. Recovering from AS therefore offers potential alternative conventional production techniques. A carrier-free, cross-linked enzyme aggregate (CLEA) was produced crude extract first time. major advantage CLEA combined immobilization, purification, stabilization into single step, thereby avoiding large amounts inert carriers final product. The contained variety demonstrated bio-conversion complex substrates.